Fast and specific dermatophyte detection by automated DNA extraction and real-time PCR
نویسندگان
چکیده
منابع مشابه
Automated extraction and quantification of human cytomegalovirus DNA in whole blood by real-time PCR assay.
The measurement of human cytomegalovirus (HCMV) DNA in blood is becoming the standard method for monitoring HCMV infection in immune-suppressed and unsuppressed patients. As various blood compartments can be used, we have compared the HCMV DNA measured in whole blood (WB), peripheral blood leukocytes (PBL), and plasma by real-time PCR. We tested 286 samples: HCMV DNA was extracted automatically...
متن کاملDetection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay
Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...
متن کاملAutomated extraction of viral-pathogen RNA and DNA for high-throughput quantitative real-time PCR.
The performance of the m1000 system (Abbott Laboratories, Illinois) as a front-end extraction system for high-throughput "in-house" quantitative real-time PCR assays was analyzed and compared to that of manual extraction of plasma and serum samples (hepatitis C virus [HCV] and hepatitis B virus [HBV]) and EDTA-blood samples (cytomegalovirus [CMV] and Epstein-Barr virus [EBV]). Linearity of extr...
متن کاملRuminant DNA detection: Real-time PCR Detection of Ruminant DNA
In order to control the spread of transmissible spongiform encephalopathy (TSE), several DNA methods have been described for the detection of the species origin of meat-andbone-meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semi-quantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of ...
متن کاملFast detection of Noroviruses using a real-time PCR assay and automated sample preparation
BACKGROUND Noroviruses (NoV) have become one of the most commonly reported causative agents of large outbreaks of non-bacterial acute gastroenteritis worldwide as well as sporadic gastroenteritis in the community. Currently, reverse transcriptase polymerase chain reaction (RT-PCR) assays have been implemented in NoV diagnosis, but improvements that simplify and standardize sample preparation, a...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2013
ISSN: 1198-743X
DOI: 10.1111/1469-0691.12153